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Between 1860 and 1864, Louis Pasteur developed the theory that microorganisms were responsible for diseases, conducted experiments that clearly indicated it, and managed to convince most of Europe that it was true. He also thought they caused infections in wounds, and suggested exposure to chemical solutions to prevent infection.
Joseph Lister, professor of surgery at the University of Glasgow, experimented with different chemical solutions and found the first real antiseptic, phenol. In 1867 he published a series of papers, and began to instruct doctors to use it.
Then he realised that infection could be better avoided by preventing bacteria from getting into wounds in the first place. He instructed surgeons under his responsibility to wear clean gloves and wash their hands before and after operations with 5% phenol solutions. Instruments were also washed in the same solution and assistants sprayed the solution in the operating theatre. This led to the rise of sterile surgery.